Technical Skills and Teaching « Karine SERRE, PhD

Technical Skills and Teaching

Biological techniques

Cellular biology:

Antigen presentation assays and measurements of T cell proliferation and IL-2 secretion by bioassay
In vitro polarisation of T helper cells (Th1, Th2, Th17, Th22 cells)
Bulk and single cell sorting by flow cytometry (Mo-Flow, FACS Aria BD)
Cytotoxic assays in vitro by the JAM test, and in vivo by adoptive transfer of CFSE labelled target cells
FACS analysis to phenotype cells (FACS Aria BD, FACS Fortessa BD, FACScalibur BD, Cyan dako)
Laser microcapture using a Microbeam HT microscope (PALM Microlaser Technologies)
Production of dendritic cells from bone marrow by the use of GM-CSF
Purification and preparation of CFSE-labelled Ag-specific transgenic CD4 or CD8 T cells, as well as Ag-specific transgenic B cells, for adoptive transfer
Tissue section analysis by immuno-histochemistry and up to 5 colors fluorescence activated confocal microscopy (Zeiss 710, Zeiss LSM510 laser scanning confocal microscope)

Molecular biology:

Biochemistry:

Immuno-technology:

Holder of a personal licence for animal experimentation:

Languages and computing knowledge

English: Fluent in spoken and written English and write my own scientific papers
Microsoft: Word, Excel, and PowerPoint
FACS analysis software: Diva, FlowJo, CELLQuest
Imaging software: AdobePhotoshop, LSM 5 Image browser
RT-PCR quantification: ViiA7, Applied Biosystem SDS 2.2
Microarrays data statistical and clustering analysis: MultiExperiment Viewer (TIGR MeV 4) software

February 4th 2014: I will participate to the teaching on “Molecular mechanisms of T cell differentiation” delivered to LisbonBioMed master student Programme. This event will involve one foreign guest, Fiona Powrie together with several Portuguese group leaders.
June 3 – 5 2013: I participated to Nanocourse “Challenges in Immunology” organised for the CAML PhD Programme. This event involved two foreign guests, Ron Germain from the NIH (USA) and Dan Pennington from Queen Mary University of London, together with several Portuguese group leaders. I gave a lecture on “Challenges in T cell differentiation”.
October – December 2009: I have given 15 tutorials for Small Group Teaching (SGT) for Medical students in the first term of their second year (2^nd year MBChB) covering Infection, Immunity and Haematology lectures
April 1999 and April 2000: Teaching on laboratory courses (practicals) for bachelor degree in Immunology on ELISA test and Immuno-electrophoresis (Université de la Méditerranée)

September 2013 (12 months): Joana Martins M.Sc. student from FCUL 1st Biochemistry program, Portugal.
April 2013 (3 months): Michael Hamm Master Student from Master Cellular and molecular biology M1 at University Pierre et Marie Curie (UPMC) Université Paris 6, France.
August 2012 (6 months): Ingrid Brok Master Student from Cell Biology and Immunology Group at the Wageningen University, The Netherlands.
February 2007 (6 months): Julie Losbtein Master Student from Ecole Supérieure de Biotechnologie de Strasbourg, France.

September 2014: Organizer of the annual meeting of the Sociedade Portuguesa de Imunologia that will occur at IMM, Lisbon, Portugal.
December 2013: I was very lucky to be asked to participate to the VII CAML PhD Students Meeting as a member of the jury selecting the best oral presentation.
October 2013: Organization of one-day workshop on “grant writing”. We had the great pleasure to welcome Dr. Judith Swan. Have a look at the advertised poster and abstract or Dr. Swan’s TEDx talk at Carnegie Mellon University in May 2013!
September 2011 – September 2012: Organization of Immunology Meetings between two institutes located in Lisboa Instituto de Medicina Molecular (IMM) and Instituto Gulbenkian de Ciência (IGC). These seminars are hosted one morning every 6 weeks with 3 talks per session.