Karine SERRE, PhD

IMMUNOLOGIST

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Latest Posts

21/01/2016

Psoriasis is an autoimmune disease and NOT a chronic inflammatory disease!

12/05/2015

CALL For PAPERS: “Innate T Cell Development and Functions in Immune Diseases”

28/08/2014

XL Annual Meeting of the Sociedade Portuguesa de Imunologia 13-15th October 2014

13/04/2013

Beginnings of breaking down the differentiation program of innate IL-17-production

27/01/2013

The improbable trio NKG2D – γδ T cells – IgE!!

More Posts

Some protocols I have gathered along the years

  1. BrdU BD kit Intracellular FACS staining
  2. Broncheolar Lavages
  3. Buffer list
  4. CFSE staining of T cells
  5. Cresyl Violet Staining for laser-microdissecting lymphoid tissue sections
  6. DNA extraction from mouse ear/tail to genotyping
  7. Experimental Autoimmune Encephalomyelitis (EAE)
  8. In vitro CD4 Th0, Th1, Th2, Th17 and Th22 cell polarizations
  9. Intracellular FACS staining paraformaldehyde with saponin
  10. Isolation of Murine Brain-infiltrating Leukocytes
  11. Isolation of Murine Hepatic Immune cells
  12. Preparation of Agarose Gel and Migration for PCR genotyping
  13. Preparation of Fetal liver cells for reconstituting chimeras
  14. Preparation Paraformaldehyde PAF 4%
  15. Staining for Transcription Factors (e-Bioscience)
  16. Thawing Cryopreserved Cells
  17. Total RNA isolation with High Pure RNA Isolation kit (Roche)
  18. Total RNA isolation with RNeasy MICRO (Qiagen)
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